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Bovine serum albumin, 40 mg/ml
 

 

Reagent name: Bovine serum albumin, 40 mg/ml

Reagent name abbreviation: BSA, 40 mg/ml

Protocol ID: PS00000019

Version: 01

Volume: 125 ml

Components:

Reagent

Source

Catalog or Protocol No.

F.W. or Stock Concentration

Quantity

Final Concentration

Bovine serum albumin (BSA)  

Sigma-Aldrich

A8806

powder

5g

40 mg/ml

 

Preparation:

1. Weigh 5 g of BSA on a balance.

2. Sprinkle BSA on top of 100 ml of purified water in a 200-ml beaker and

let it dissolve without agitation.

3. Transfer solution to a 200 ml graduated cylinder. Use purified water to

rinse the beaker and adjust the volume to 125 ml.

4. Cover cylinder with Parafilm and mix by inverting gently three times.

5. Divide into 0.26 ml aliquots in 1.5-ml Eppendorf tubes.

Storage:

Temperature: -80°C

Location: -80°C freezer

Aliquot size: 0.26 ml

Special instructions: NA

Author:

Date:    

Approved:

 

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