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Bovine serum albumin, 5%, in Tris-buffered saline Tween 20, 0.1%
 

 

Reagent name: Bovine serum albumin, 5%, in Tris-buffered saline Tween 20, 0.1%

Reagent name abbreviation: 5% BSA/0.1% TBST

Protocol ID: PS00000020

Version: 01

Volume: 500 ml

Components:

Reagent

Source

Catalog or Protocol No.

F.W. or Stock Concentration

Quantity

Final Concentration

Bovine serum albumin (BSA) (fraction V)

Fisher Biotech

powder

BP1605-100

25 g

5% (w/v)

Tris-buffered saline pH 7.6, 10X (10X TBS)

None

PS00000062

10X

50 ml

1X

 

OmniPur

Polyoxyethylene (20)

monolaurate

(Tween 20)

EM Science

9480

1164

500 µl

0.1% (v/v)

 

,

Preparation:

1. Pour 400 ml of purified water into a beaker and stir.

2. Add 10X TBS and Tween 20. Be sure to rinse all of the Tween 20 from the pipette tip

into the solution.

3. Add BSA, cover with plastic wrap, and stir until completely dissolved.

4. Transfer solution to a graduated cylinder and adjust to final volume with purified

water.

5. Cover cylinder with Parafilm and mix by inversion.

6. Filter solution using a Nalgene SFCA filter unit (0.45 µm pore size).

Storage:

Temperature: 4 °C

Location: __________________

Aliquot size: NA

Special instructions: None

Author:

Date:    

Approved:

Comments:

 

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