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Bovine serum albumin
 

Reagent name: Bovine serum albumin, 100 mg/ml

Reagent name abbreviation: MC BSA

Protocol ID: PS00000445

Version: 02

Volume: 50 ml

Components:

Reagent

Source

Catalog or Protocol No.

F.W. or Stock Concentration

Quantity

Final Concentration

Bovine serum

albumin (BSA)

Sigma-Aldrich

A8806

NA

5g

100 mg/ml

 

Preparation:

1. Weigh BSA and add to 30 ml purified water in a 100-ml beaker.

2. Stir until BSA is thoroughly dissolved.

3. Pour solution into a graduated cylinder and adjust volume to 50 ml with purified

water.

4. Cover solution with Parafilm and invert to mix.

5. Prewash a 0.2 µM filter attached to a 60-ml syringe with 10 ml of purified water.

6. Sterilize the solution by filtering through the prewashed filter.

7. Pipette sterilely into 5 ml aliquots.

Storage:

Temperature: -20 °C

Location: __________________

Aliquot size: 5 ml

Special instructions: None

Author:

Date:    

Approved:

Comments:

 

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