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Myocyte culture medium
 

Reagent name: Myocyte culture medium

Reagent name abbreviation: MC culture medium

Protocol ID: PS00000446

Version: 02

Volume: 500 ml

Components:

Reagent

Source

Catalog or Protocol No.

F.W. or Stock Concentration

Quantity

Final Concentration

Minimum essential

medium (MEM), 1X

(with Hanks’ salts

and L-glutamine)

 

GIBCO/

Invitrogen

11575032

1X

490 ml

1X

 

Bovine serum

albumin, 100 mg/ml

 

None

 

PS00000445

 

100 mg/ml

 

500 µl

 

0.1 mg/ml

 

Penicillin

 

None

 

PS00000453

 

10,000 U/ml

 

5 ml

 

100 U/ml

 

L-Glutamine

 

 

25030149

 

200 mM

 

5 ml

 

2 mM

 

Preparation:

1. Sterilely remove 10.5 ml of medium from a fresh bottle of MEM.

2. Measure and add BSA, penicillin, and glutamine to remaining MEM.

3. Cap bottle and swirl to mix thoroughly.

Storage:

Temperature: 4 °C

Location: __________________

Aliquot size: NA

Special instructions: Equilibrate medium for 2 to 3 hr in a 2% CO2 incubator at 37 °C

prior to use. This will adjust temperature and pH balance of the medium. Final pH

should be approximately 7.0.

Author:

Date:    

Approved:

Comments:

 

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