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Myocyte plating medium
 

Reagent name: Myocyte plating medium

Reagent name abbreviation: MC plating medium

Protocol ID: PS00000448

Version: 02

Volume: 500 ml

Components:

Reagent

Source

Catalog or Protocol No.

F.W. or Stock Concentration

Quantity

Final Concentration

Minimum essential

medium (MEM), 1X (with

Hanks’ salts and

L-glutamine)

 

GIBCO/

Invitrogen

 

11575032

 

1X

 

455 ml

 

0.9X

 

Bovine calf serum (BCS)

 

HyClone

 

SH30073

 

100%

 

25 ml

 

5%

 

2,3-Butanedione

monoxime (BDM),

500 mM

 

None

 

PS00000438

 

500 mM

 

10 ml

 

10 mM

 

Penicillin

 

None

 

PS00000453

 

10,000 U/ml

 

5 ml

 

100 U/ml

 

L-Glutamine

 

GIBCO/

Invitrogen

 

25030149

 

200 mM

 

5 ml

 

2 mM

 

Preparation:

1. Sterilely remove 45 ml of medium from a fresh bottle of MEM using a 50-ml pipette.

2. Sterilely measure and add BCS, BDM, penicillin, and glutamine to remaining MEM for a final volume of 500 ml.

3. Cap bottle and swirl to thoroughly mix.

Storage:

Temperature: 4 °C

Location: __________________

Aliquot size: NA

Special instructions: Solution may be stored for up to 4 days. Equilibrate medium for 2 to 3 hr in a 2% CO2 incubator at 37 °C prior to use. This will adjust temperature and pH balance of the medium. Final pH should be approximately 7.0.

Author:

Date:       

Approved:

Comments:

 

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