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Myocyte stopping buffer 1
 

 

Reagent name: Myocyte stopping buffer 1

Reagent name abbreviation: MC stop 1

Protocol ID: PS00000449

Version: 01

Volume: 10 ml

Components:

Reagent

Source

Catalog or Protocol No.

F.W. or Stock Concentration

Quantity

Final Concentration

Minimum Perfusion buffer

 

None

 

PS00000451

 

1X

 

9 ml

 

1X

 

Bovine calf serum (BCS)

 

HyClone

 

SH30073

 

100%

 

1 ml

 

10%

 

Calcium chloride

(CaCl2), 10 mM

 

None

 

PS00000440

 

100 mM

 

12.5 µl

 

12.5 µM

 

 

Preparation:

1. Pipette 1 ml of BCS and 12.5 µl of CaCl2 into a 10-ml graduated cylinder.

2. Adjust final volume to 10 ml with perfusion buffer.

3. Cover with Parafilm and mix by inversion.

4. Prewash a 50-ml tube filter with 10 ml purified water.

5. Sterilize the solution by filtering through the prewashed filter.

Storage:

Temperature: NA

Location: __________________

Aliquot size: NA

Special instructions: Do not store solution. Prepare fresh as needed.

Author:

Date:         

Approved:

Comments:

 

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