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Myocyte stopping buffer 2
 

 

Reagent name: Myocyte stopping buffer 2

Reagent name abbreviation: MC stop 2

Protocol ID: PS00000450

Version: 01

Volume: 30 ml

Components:

Reagent

Source

Catalog or Protocol No.

F.W. or Stock Concentration

Quantity

Final Concentration

Perfusion buffer

 

None

 

PS00000451

 

1X

 

28.5 ml

 

1X

 

Bovine calf serum (BCS)

 

HyClone

 

SH30073

 

100%

 

1.5 ml

 

10%

 

Calcium chloride

(CaCl2), 10 mM

 

None

 

PS00000440

 

10 mM

 

37.5 µl

 

12.5 µM

 

 

Preparation:

1. Pipette 1.5 ml of BCS and 37.5 µl of CaCl2 into a 50-ml graduated cylinder.

2. Adjust final volume to 30 ml with perfusion buffer.

3. Cover with Parafilm and mix by inversion.

4. Prewash a 0.2 µM filter attached to a 35-ml syringe with 10 ml of purified water.

5. Sterilize the solution by filtering through the prewashed filter.

Storage:

Temperature: NA

Location: __________________

Aliquot size: NA

Special instructions: Do not store solution. Prepare fresh as needed.

Author:

Date:       

Approved:

Comments:

 

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