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Perfusion buffer
 

 

 

Reagent name: Perfusion buffer, pH 7.46

Reagent name abbreviation: Perf buf

Protocol ID: PS00000451

Version: 01

Volume: 500 ml

Components:

Reagent

Source

Catalog or Protocol No.

F.W. or Stock Concentration

Quantity

Final Concentration

Stock perfusion

buffer

 

None

 

PS00000452

 

1X

 

490 ml

 

.98X

 

2,3-Butanedione

monoxime (BDM),

500 mM

 

None

 

PS00000438

 

500 mM

 

10 ml

 

 

10 mM

 

Glucose

 

Sigma-Aldrich

 

G7528

 

180.2 g/mol

 

0.5 g

 

5.5 mM

 

 

Preparation:

1. Weigh glucose and add to 400 ml of stock perfusion buffer in a 1-L beaker.

2. Add 10 ml of BDM to the beaker and stir until all components are thoroughly

dissolved.

3. Transfer solution to a 500-ml graduated cylinder and adjust final volume to 500 ml with stock perfusion buffer.

4. Cover with Parafilm and mix by inversion.

5. Prewash a 0.22-µM 500-ml filter unit with 10 ml purified water.

6. Sterilize the solution by filtering through the prewashed filter unit.

7. Final pH is 7.46 after filtration.

Storage:

Temperature: NA

Location: __________________

Aliquot size: NA

Special instructions: Do not store solution. Prepare fresh as needed.

Author:

Date:     

Approved:

Comments:

 

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