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Ringer’s solution (calcium-free), pH 7.4
 

 

 

Reagent name: Ringer’s solution (calcium-free), pH 7.4, 1X

Reagent name abbreviation: 1X Ringer’s solution (NO Ca2+), pH 7.4

Protocol ID: PS00000425

Version: 01

Volume: 500 ml

Components:

Reagent

Source

Catalog or Protocol No.

F.W. or Stock Concentration

Quantity

Final Concentration

Ringer’s stock, 10X

 

None

 

PS00000431

 

10X

 

50 ml

 

1X

 

D-(+)-glucose

None

 

PS00000421

 

1 M

 

5 ml

 

10 mM

 

Magnesium chloride

(MgCl2)

None

 

PS00000415

 

0.1 M

 

10 ml

 

2 mM

 

Sodium hydroxide

(NaOH)

 

None

 

PS00000038

 

1 N

 

Titrated

 

NA

 

Preparation:

1. Pour 300 ml of purified water into a 500-ml beaker.

2. Add 10X Ringer’s stock, D-(+)-glucose, and calcium chloride, and stir to mix.

3. Adjust pH to 7.4 with 1 N NaOH.

4. Adjust volume to 500 ml with purified water in a graduated cylinder.

5. Sterilize with 0.2-µm filter.

Storage:

Temperature: 4 °C

Location: __________________

Aliquot size: NA

Special instructions: None

Author:

Date:        

Approved:

Comments:

 

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