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Ringer’s solution
 

 

Reagent name: Ringer’s solution, 10X

Reagent name abbreviation: 10X Ringer’s solution

Protocol ID: PS00000431

Version: 01

Volume: 500 ml

Components:

Reagent

Source

Catalog or Protocol No.

F.W. or Stock Concentration

Quantity

Final Concentration

Potassium chloride

(KCl)

 

None

 

PS00000424

 

2.5 M

 

4 ml

 

20 mM

 

Potassium

phosphate (K2HPO4)

 

None

 

PS00000423

 

1 M

 

7.5 ml

 

15 mM

 

Magnesium sulfate

(MgSO4)

 

None

 

PS00000414

 

1 M

 

5 ml

 

10 mM

 

HEPES

 

None

 

PS00000399

 

1 M

 

50 ml

 

100 mM

 

Sodium chloride

(NaCl)

 

Sigma-Aldrich

 

S9888

 

58.44

 

40.91 g

 

1.4 M

 

Preparation:

1. Pour 300 ml of purified water into a 500-ml beaker.

2. Add components and stir to mix.

3. Adjust volume to 500 ml with purified water in a graduated cylinder.

4. Sterilize with 0.2-µm filter.

Storage:

Temperature: 4 °C

Location: __________________

Aliquot size: NA

Special instructions: None

Author:

Date:        

Approved:

Comments:

 

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