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Stock perfusion buffer
 

 

Reagent name: Stock perfusion buffer, 1X

Reagent name abbreviation: 1X Perf buf stock

Protocol ID: PS00000452

Version: 01

Volume: 1 L

Components:

Reagent

Source

Catalog or Protocol No.

F.W. or Stock Concentration

Quantity

Final Concentration

Sodium chloride

(NaCl)

Sigma-Aldrich

 

S9625

 

58.4 g/mol

 

6.6 g

 

113 mM

 

Potassium chloride

(KCl)

Sigma-Aldrich

 

P4504

 

74.6 g/mol

 

0.35 g

 

4.7 mM

 

Potassium

phosphate

monobasic

(KH2PO4)

Sigma-Aldrich

 

P5379

 

136.1 g/mo

 

0.082 g

 

0.6 mM

 

Sodium phosphate

dibasic (Na2HPO4)

 

Sigma-Aldrich

 

S0876

 

142 g/mol

 

0.085 g

 

0.6 mM

 

Magnesium sulfate

heptahydrate

(MgSO4-7H20)

Sigma-Aldrich

 

M9397

 

246.5 g/mol

 

0.3 g

 

1.2 mM

 

Phenol red

 

Sigma-Aldrich

 

P5530

 

376.4 g/mol

 

0.012 g

 

0.032 mM

 

Sodium bicarbonate

(NaHCO3)

Sigma-Aldrich

 

S5761

 

84 g/mol

 

1.01 g

 

12 mM

 

Potassium

bicarbonate

(KHCO3)

Sigma-Aldrich

 

P9144

 

101 g/mol

 

1.01 g

 

10 mM

 

HEPES buffer

solution, 1 M

GIBCO/

Invitrogen

15630080

 

1 M

 

10 ml

 

10 mM

 

Taurine

 

Sigma-Aldrich

T0625

 

125.1 g/mol

 

3.75 g

 

30 mM

 

Preparation:

1. Add all components to 900 ml purified water in a 2-L beaker.

2. Stir until all components are thoroughly dissolved.

3. Transfer to a 1-L graduated cylinder and adjust final volume to 1 L with purified water.

4. Prewash a 0.22-µM filter unit with 10 ml purified water.

5. Sterilize the solution by filtering through the prewashed filter unit.

Storage:

Temperature: 4 °C

Location: __________________

Aliquot size: NA

Special instructions: Solution may be stored for up to 1 week.

Author:

Date:        

Approved:

Comments:

 

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